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[ sedfitdownload ] getting started ] examples ] [ tutorials ] sedfit help ] FAQs ] references ] links ] contact ] Search ] [ sedphat ]

tutorials

For a relatively recent video tutorial on c(s), see https://sedfitsedphat.nibib.nih.gov/tools/Tutorials/Forms/AllItems.aspx

 

Because the use of Sedfit requires some theoretical background on sedimentation analysis, there are websites with introductions in 

    * Lamm equation solutions 

    * systematic noise analysis 

    * size-distribution analysis 

    * ls-g*(s) 

    * van Holde-Weischet analysis

    * Compressibility of water and organic solvents

    * Sedimentation in dynamic self-forming density gradients

    * generating simulated data

 

There is also a step-by-step tutorial on how to use Sedfit

You can also consult the step-by-step protocol https://www.nature.com/articles/nprot.2017.064 (this is using fluorescence optical data, but the data analysis applies equally to other optical systems).

As documentation please refer to the following books, which have specific information on SEDFIT and SEDPHAT functions as side-bars nested into the description of theory and experiment of analytical ultracentrifugation (AUC):

https://www.crcpress.com/Basic-Principles-of-Analytical-Ultracentrifugation/Schuck-Zhao-Brautigam-Ghirlando/p/book/9781498751155

https://www.crcpress.com/Sedimentation-Velocity-Analytical-Ultracentrifugation-Discrete-Species/Schuck/p/book/9781498768948

https://www.crcpress.com/Sedimentation-Velocity-Analytical-Ultracentrifugation-Interacting-Systems/Schuck-Zhao/p/book/9781138035287

 Note also the Experimental Protocols website for how to plan, conduct, and plan analysis of AUC experiments.