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This mirrors files containing experimental protocols developed at the National Institutes of Health.
The protocols contain detailed step-by-step information on how to plan, set-up and analyze experiments.
For more specific and up-to-date information please refer to the following books, which have specific information on SEDFIT and SEDPHAT functions as side-bars nested into the description of theory and experiment of analytical ultracentrifugation (AUC):
You can also consult the step-by-step protocol https://www.nature.com/articles/nprot.2017.064 (this is using fluorescence optical data, but the data analysis applies equally to other optical systems).
Flow Chart for AUC Experiments
Criteria for Buffer Selections
Grid for SV and SE Run Conditions when Using Different Optical Systems
Typical Strategies for Common Problems